Molecular and Histopathology Core Services

Learn more about the services provided by the Molecular and Histopathology Core.

Routine Histology – Processing, Embedding, Sectioning, and Hematoxylin and Eosin Staining

Routine Histology microscopic image

  • Fixation and processing of human and animal tissues, as well as cultured cells and tissues Paraffin embedding and sectioning of tissues
  • Embedding and cryostat sectioning of fresh, frozen tissues
  • Routine H&E staining of human and animal tissues, as well as cultured cells and tissues
  • H&E staining for paraffin or frozen sections

Histochemical Stains

Histochemical Stains microscopic image

  • Histochemical staining of paraffin or frozen tissue sections
  • PAS
  • Masson Trichrome
  • Toluidine Blue
  • Gomori’s Aldehyde Fuchsin
  • Safranin O
  • Verhoeff Elastic
  • Cresyl Violet
  • Ability to develop and optimize histochemical stains for any tissue type or staining need

Immunohistochemical Stains

Immunohistochemical Staining microscopic image

  • Immunohistochemical staining oh Human and animal tissues, as well as cultured cells and tissues
  • Ability to validate and develop protocols for any antibody
  • Mouse-on-mouse/rat immunohistochemistry
  • Chromogenic and fluorescent detection methods
  • Dual and triple IHC techniques
  • All IHC performed on an automated Ventana Discovery XT stainer

In situ Hybridization

In Situ Hybridization microscopic image

  • Chromogenic or fluorescent in situ hybridization protocols
  • Ability to develop and optimize protocols for your specific probes
  • All hybridizations protocols performed on an automated Ventana Discovery XT stainer

Tissue Microarray

Tissue Microarray microscopic image

  • Paraffin tissue microarrays (TMAs) can be created with any combination of human tissue, animal tissues
    or cell culture pellets
  • Custom-sized and configured arrays are constructed using the Beecher MTA-I
  • Tissue core sizes can range from 0.6 mm to 2.0 mm, providing from 40 to over 200 sample cores in one
    paraffin block
  • Microarray slides can be stained using a variety of histochemical or immunohistochemical reactions

Photomicroscopy

Photomicroscopy microscopic image

  • Create digital light microscopic images of your stained slides for presentation or publication
  • Microscope system includes an Olympus BX51 microscope outfitted with a DP25 digital camera
  • Ability to photography from 4x-60x
  • Photo editing software also available for minor image corrections

Laser Capture Microdissection

Laser Capture Microdissection microscopic image

  • Zeiss PALM Microbeam LCM
  • Capable of isolating cells, tissues, or stromal matrix from either frozen and paraffin sections
  • Ideal method to insure the purity of the starting material for downstream proteomic and genomic applications
  • Capable of operating on an RNAse free level to insure high quality samples for genomic studies

Archival FFPE Human Tissue Samples

Archival FFPE Human Tissue Bank image

  • Archival collection consisting of over 40 years of surgical pathology FFPE specimens
  • Searchable database enables the investigator to locate specific patient, tissue or tumor types
  • Ability to collaborate with anatomic pathologists to assist in the identification and selection of study samples along with the interpretation and scoring of results
  • Perfect for use as control tissues, study samples or TMA sources
  • Capable of isolating DNA or RNA from samples

Contact Us

For all technical and project-related questions about the Molecular and Histopathology Core, please contact Marianne Klinger, Research Technician, at mklinger@pennstatehealth.psu.edu or 717-531-1044.

For lab management questions, please contact Douglas B. Stairs, PhD, Director, at dbs18@psu.edu or 717-531-6725.