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Macromolecular Synthesis

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The Macromolecular Synthesis Core at Penn State College of Medicine was established in 1987, with primary grant support from the National Science Foundation and College of Medicine funds. The facility subsequently received grant support from the NIH and the Commonwealth of Pennsylvania via the Ben Franklin Partnership, and matching funds from the College of Medicine.

Services offered include:

  • Peptide synthesis
  • Oligonucleotide synthesis
  • Image analysis and gel band quantitation (for which instrumentation purchased with Tobacco Settlement Funds)

Submit peptide synthesis request form Submit oligosynthesis request form

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Instrumentation and Services

Peptide Synthesis Expand answer

A standard synthesis delivers between 50 and 80 mg of peptide. Custom syntheses are also possible (e.g., phosphopeptides, biotinylated peptides), with an additional cost being determined by the reagents used.

Oligosynthesis Expand answer

The Polygen synthesizer is used to generate oligonucleotides. The cost depends on the scale of the synthesis needed. Many users choose to have their oligonucleotides purified, which can be done with Nensorb columns. Custom syntheses such as modified bases and phosphorothioate chemistries are also available, as are larger-scale syntheses.

Procedures, Protocols and Forms

Peptide Synthesis Procedures Expand answer

Full-scale synthesis (approx. 0.2 mmol yield) or half-scale synthesis (approx. 0.1 mmol yield) is available, with fees that cover the cost of the reagents.

Peptides will be prepared as a lyophilized powder cleaved from the resin in the presence of appropriate side group preservatives, extensively washed and extracted following cleavage with various solvents to remove the majority of the impurities (preservatives and byproducts of the cleavage procedure.

Molecular weight verification and synthesis purity is available using mass spectrometry for an additional fee.

To place an order, complete the Peptide Synthesis Request Form.

Oligosynthesis Procedures Expand answer

The core can provide 0.05 µM scale synthesis, 0.25 µM scale synthesis or 1.0µM scale synthesis.

Two different levels of Nensorb purification are available: one for oligos synthesized at 0.05 µM or 0.2 µM scale synthesis, and another larger column used for oligos synthesized at 1.0µM scale synthesis.

Pickup procedure: After synthesis, oligos may be picked up from the top shelf of the freezer in room C1732, with the data sheets attached to the side of the freezer. An approximate yield of micrograms of oligo will be included on the data sheet, but because this is calculated based on the A260 while the oligo is still in methanol before lyophilization, it is only approximate. Investigators must requantitate the yield and concentration of each oligo after it is resuspended in water or TE buffer in order to have an accurate measure of yield/concentration.

To place an order, complete the Oligosynthesis Request Form.

Citation Expand answer

All publications, press releases or other documents that result from the utilization of any Penn State College of Medicine Institutional Research Resources, including funding, tools, services or support, are required to credit the core facility and associated RRID.

“The Macromolecular Synthesis Core (RRID:SCR_017831) services and instruments used in this project were funded, in part by the Pennsylvania State University College of Medicine via the Office of the Vice Dean of Research and Graduate Students and the Pennsylvania Department of Health using Tobacco Settlement Funds (CURE). The content is solely the responsibility of the authors and does not necessarily represent the official views of the university or College of Medicine.”

Sample Shipping Expand answer

All samples must be shipped to this address:

Macromolecular Synthesis Core
Penn State College of Medicine
500 University Dr.
Room C1732
Hershey, PA 17033

Work With Macromolecular Synthesis

Fees Expand answer

For pricing on any services, including non-standard amino acids and non-standard nucleotides, or other specialized preparation procedures that may be necessary for a particular research purpose, call the core at 717-531-6087 or email Anne Stanley at astanley@psu.edu or Suja Maddukuri at srm25@psu.edu.

Contact the Core Expand answer